RNase I (DNase free)

SKU: orb532705

Description

Ribonuclease l (27 kD) is a completely nonspecific ribonuclease that hydrolyzes the phosphodiester bond of all four bases. It degrades any RNA to a mixture of mono-, di-, and trinucleotides and does not degrade DNA, although it does bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in RNase Protection Assays. It has a high specific activity which, coupled with its non-specificity, typically results in complete degradation of RNA using ng amounts of protein. Contains no endonuclease or exonuclease activity toward DNA substrates.

Images & Validation

• Application Notes:
  degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate. Ribonuclease Protection Assays. Mapping or Quantitation of RNA by selective cleavage of single-strand regions. Eliminates RNA from DNA-and protein purification.

Key Properties

• Molecular Weight: Theoretical MW: 27.0 kDa
• Purity: > 95% (SDS-PAGE)

Storage & Handling

• Storage: Store at -20 °C. avoid freeze/thaw cycles.
• Form/Appearance: liquid (Supplied in 10 mM Tris-HCl pH 8.0, 200 mM NaCl and 50 % [v/v] glycerol)
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Endonuclease, recombinant, E. coli