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RIPK1 Rabbit Polyclonal Antibody (BF647)
Catalog Number: orb2408439
| Catalog Number | orb2408439 |
|---|---|
| Category | Antibodies |
| Description | RIPK1 Rabbit Polyclonal Antibody (BF647) |
| Target | RIPK1 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | BF647 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Bovine, Equine, Porcine, Rabbit |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human RIPK1 (581-671/671aa) |
| Tested applications | IF |
| Dilution range | IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Alternative names | Cell death protein RIP; Receptor (TNFRSF) interact Read more... |
| Note | For research use only |
Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-RIPK-1 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-RIPK-1 /AF647 Conjugated antibody (orb2408439), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-AF647. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
