RIP3 Antibody [Rippy-3]

• Catalog Number: orb1273948
• Category: Antibodies
• Description: RIP3 Antibody [Rippy-3]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Rippy-3
• Tested applications: WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: RIP3 (monoclonal Rippy-3) antibody was raised against recombinant human RIP3.
• Concentration: batch dependent
• Dilution range: RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: Q9Y572
• Storage: RIP3 antibody can be stored at 4°C, stable for one year.
• Buffer/Preservatives: PBS containing 0.02% sodium azide
• Note: For research use only
• Application notes: RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only.
• Expiration Date: 12 months from date of receipt.