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Catalog Number | orb1273948 |
---|---|
Category | Antibodies |
Description | RIP3 Antibody [Rippy-3] |
Clonality | Monoclonal |
Isotype | IgG1 |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | PBS containing 0.02% sodium azide |
Purification | RIP3 (monoclonal Rippy-3) antibody was purified to > =95% in SDS-PAGE. |
Immunogen | RIP3 (monoclonal Rippy-3) antibody was raised against recombinant human RIP3. |
UniProt ID | Q9Y572 |
Tested applications | WB |
Application notes | RIP3 antibody can be used to recognize human RIP3 by western blot. Method: HEK 293T cells (5x105) mock transfected (lane 1) or transfected (lane 2) with an expression plasmid coding for human RIP3 were harvested, lysed and cell extracts (5x105) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the Rippy-3 monoclonal antibody at a 1:1000 dilution for 2 hours. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG and a chemiluminescence detection system. This product is for research use only. |
Antibody Type | Primary Antibody |
Clone Number | Rippy-3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |