RHOXF2B Antibody (Center)

SKU: orb1788000

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-141 amino acids from the Central region of human RHOXF2B. Antigen Region: 110-141 aa.
• Target: RHOXF2B (HGNC:33519)
• Molecular Weight: 31637 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rhox homeobox family member 2B, RHOXF2B

All lanes: Anti-RHOXF2B Antibody (Center) at 1:1000 dilution. Lane 1: A549 whole cell lysates. Lane 2: K562 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.

Quick Database Links

Gene Symbol

RHOXF2B (HGNC:33519)

UniProt

P0C7M4