RHOXF2B Antibody (Center)

• Catalog Number: orb1788000
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This RHOXF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-141 amino acids from the Central region of human RHOXF2B.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: 110-141 aa
• UniProt ID: P0C7M4
• MW: 31637 Da
• Tested applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Rhox homeobox family member 2B, RHOXF2B
• Note: For research use only

All lanes: Anti-RHOXF2B Antibody (Center) at 1:1000 dilution. Lane 1: A549 whole cell lysates. Lane 2: K562 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly.