RHOT1 Rabbit Polyclonal Antibody

• Catalog Number: orb579298
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RHOT1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RHOT1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 71 kDa
• Target: RHOT1
• UniProt ID: Q8IXI2
• Protein Sequence: Synthetic peptide located within the following region: EMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDV
• NCBI: NP_001028738
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ARHT1, MIRO1, MIRO-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~56-54 kDa.

WB Suggested Anti-RHOT1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 293T cell lysate. RHOT1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.