RHO Antibody

• Catalog Number: orb1672040
• Category: Antibodies
• Description: RHO Antibody
• Clonality: Recombinant
• Clone Number: Rho 1D4
• Tested applications: ELISA, IF, IHC, IP, RIA, WB
• Reactivity: Amphibian, Bovine, Human, Mouse, Rat, Zebrafish
• Isotype: IgG kappa
• Immunogen: Bleached bovine rod outer segment (ROS).
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: RHO
• UniProt ID: P08100
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Rho-tag, Visual Purple
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-Rhodopsin antibody Rho 1D4. Rat eye lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of Rho 1D4 (orb1672040) at 0.0001 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat (Rattus norvegicus) Rhodopsin is 39.0kDa- tho ugh this protein in known to form dimers and higher order multimers (c.f. Mansoor et al.- PMID: 16492772- PMID: 10798675). orb1672040 successfully detected monomeric- dimeric and multimeric Rhodopsin in rat eye lysate.