RHD Antibody

• Catalog Number: orb1671729
• Category: Antibodies
• Description: RHD Antibody
• Clonality: Recombinant
• Clone Number: D7C2
• Tested applications: Cytotoxic Assay, FC
• Reactivity: Human
• Isotype: IgG lambda
• Immunogen: A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: RHD
• UniProt ID: Q02161
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD240D, RHXIII, Rh polypeptide 2, RhPII
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed K562 cells with anti-Rhesus D antigen antibody D7C2. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of D7C2 (orb1671729) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671729- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-Rhesus D antigen antibody D7C2. Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of D7C2 (orb1671729 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.