RHAMM Antibody

• Catalog Number: orb1239142
• Category: Antibodies
• Description: RHAMM Antibody
• Target: HMMR
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: RHAMM Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: RHAMM Antibody is affinity chromatography purified via peptide column.
• Immunogen: RHAMM antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RHAMM.The immunogen is located within amino acids 80 - 130 of RHAMM.
• UniProt ID: O75330
• Tested applications: ELISA, IF, IHC-P, WB
• Application notes: RHAMM antibody can be used for detection of RHAMM by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RHAMM Antibody: CD168, IHABP, RHAMM, Hyaluronan me
• Research Area: Cancer Biology, Cell Biology
• Note: For research use only
• NCBI: NP_036617

RHAMM KO Validation in HeLa Cells, Loading: 10 µg of lysate Antibodies: XRHAMM orb1239142, 1 µg/mL and beta-actin, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Western blot analysis of RHAMM in rat stomach tissue lysate with RHAMM antibody at 0.5 µg/mL.

Immunohistochemistry of RHAMM in human stomach tissue with RHAMM antibody at 2.5 µg/mL.

Immunofluorescence of RHAMM in human stomach tissue with RHAM antibody at 20 µg/mL.