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rGFP Protein
SKU: orb81316
Description
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Escherichia Coli |
|---|---|
| Protein Sequence | MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPEGYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLADHYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
|---|---|
| Form/Appearance | Sterile filtered yellowish solution. |
| Buffer/Preservatives | The Green Fluorescent Protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8 and 10% Glycerol. |
| Disclaimer | For research use only |
Alternative Names
−Green fluorescent protein, GFP.
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GFP Control protein [orb345957]
SDS-PAGE, WB
Unconjugated
GFP control protein was synthesized with an amino terminal 6XHIS tag and was expressed in E. coli and purified by sequential hydrophobic interaction chromatography, DEAE chromatography and size-exclusion chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-GFP. Analysis by SDS-PAGE resulted in a pattern consistent with purified GFP and was estimated to be greater than 90% pure.
100 μg
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rGFP Protein (orb81316)
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