RFX5 Antibody

SKU: orb344607

Description

RFX5 antibody

Images & Validation

• Tested Applications: ELISA, Gel Supershift Assays, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, Gel Supershift Assays: 0.5 µL to 1.0 µL, WB: 1:1,000
• Reactivity: Human
• Application Notes:
  Anti RFX5 antibody has been assayed by immunoblot and found to be reactive against RFX5 (aa 320 to 494) from a variety of fibroblast and B-cell lysates at a dilution of 1:1,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 µl per assay.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: RFX5
• Purity: RFXF5 antibody was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-RFX5 antibody, DNA binding protein RFX 5 antibody, Influences HLA class II expression antibody, Regulatory factor X 5 antibody, Regulatory factor X subunit 5 antibody, Regulatory factor X 5 (influences HLA class II expression) antibody

Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Hela Nuclear Extract. Load: 25 µg per lane. Primary antibody: RFX5 antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 65.3 kDa, 60 kDa for RFX5.

Western Blot of Rabbit Anti-RFX5 antibody. Lane 1: Raji B cell nuclear extract lysates. Lane 2: none. Load: 35 µg per lane. Primary antibody: RFX5 antibody at 1:2500 for overnight at 4°C. Secondary antibody: HRP Goat-a-Rabbit IgG secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 65.3 kDa, 72 kDa for RFX5. Other band(s): none.

Quick Database Links

Gene Symbol

RFX5

UniProt

P48382

RefSeq:CAO72162.1