RFP Rabbit Polyclonal Antibody

SKU: orb1499430

Description

RFP Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant Red fluorescent protein
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Red fluorescent protein; Red fluorescent protein eqFP611; RFPTag; RFP-Tag; RFP Tag; RFP_ENTQU.

Measured by its binding ability in a indirect ELISA. Immobilized Human RFP protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human RFP Antibody, the EC50 is 147.7 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human RFP protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human RFP Antibody, the EC50 is 161.8 ng/ml.

Sample: Lane 1: Recombinant RFP protein, His, Lane 2: Tags Positive Control Whole Cell Lysate, C-His, Primary: Anti-RFP (orb1499430) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 30, 44 kDa.