RFP protein

SKU: orb345960

Description

RFP protein

Images & Validation

• Tested Applications: DOT, SDS-PAGE, WB
• Application Notes:
  RFP Control is suitable as a control for polyclonal or monoclonal Anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa. Recombinant RFP has been tested in SDS-page, dot blot, and western blot.

Key Properties

• Expression System: E. coli
• Purity: Recombinant RFP was synthesized with an amino terminal 6XHIS tag and was expressed in E. coli. A 3 step process was used to purify the protein: a partitioning separation using t-butanol, followed by hydrophobic interaction chromatography (HIC), and lastly IMAC nickel chromatography. Analysis by SDS-PAGE resulted in a pattern consistent with purified RFP and was estimated to be greater than 90% pure.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: None
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

RFP control protein, DsRed protein, rDsRed control, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

SDS-PAGE of purified RFP control shows a single band approximately 28-30 kDa (arrow). Approximately 1 µg of purified protein was loaded on to a 4-20% gradient gel for separation followed by staining with Coommassie blue. Molecular weight estimation was made by comparison to MW markers indicated at the right.

Western blot using RFP control protein. Lane 1: Super Signal Molecular Weight. Lane 2: 50 ng of RFP (p/n orb345960). Primary Antibody: Rabbit Anti-RFP (p/n orb345391) at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) 1:40000 for 30 mins at RT. Expect ~27kDa.

Quick Database Links

UniProt

Q9U6Y8