RFP antibody

• Catalog Number: orb345910
• Category: Antibodies
• Description: RFP antibody
• Species/Host: Gallus
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Other
• Isotype: IgY
• Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000, WB: 1:1,000 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot.
• Conjugation: Unconjugated
• UniProt ID: Q9U6Y8
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: chicken anti-RFP antibody, DsRed, rDsRed, Discosom
• Note: For research use only
• Application notes: Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA, SDS-PAGE, and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
• Expiration Date: 12 months from date of receipt.

References

Kivelä, Riikka et al. The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation Nat Commun, 7, 13124 (2016)

PubMed: 27731315

Western blot analysis of nitrocellulose membrane lysates using RFP antibody