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Catalog Number | orb1264585 |
---|---|
Category | Antibodies |
Description | RFC5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, WB |
Reactivity | Human, Rat |
Isotype | Rabbit Ig |
Immunogen | This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human RFC5. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 38 kDa |
Target | RFC5 |
UniProt ID | P40937 |
NCBI | P40937 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Replication factor C subunit 5, Activator 1 36 kDa Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti in Hela cell line lysates (35 ug/lane)
Western blot analysis of anti in rat PASMC cell line lysates (35 ug/lane).
Western blot analysis of RFC5 using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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