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RFC5 Antibody (Center R185)
SKU: orb34152
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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 4
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB: 1:1000, FACS: 1:10-50 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB17319 |
| Immunogen | Synthetic Peptide |
| Target | RFC5 |
| Molecular Weight | 38497 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-Replication factor C subunit 5 antibody, anti-Activator 1 subunit 5 antibody, anti-Replication factor C 36 kDa subunit antibody, anti-RF-C 36 kDa subunit antibody, anti-RFC36 antibody, anti-Activator 1 36 kDa subunit antibody, anti-A1 36 kDa subunit antibody, anti-RFC5 antibody
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Western blot analysis of anti-RFC5 Antibody (Center R185) in Hela cell line lysates (35 ug/lane). RFC5 (arrow) was detected using the purified Pab.
Western blot analysis of anti-RFC5 Antibody (Center R185) in rat PASMC cell line lysates (35 ug/lane). RFC5 (arrow) was detected using the purified Pab.
Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.
RFC5 Antibody (Center R185) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_853556.2, NP_001123584.1, NP_031396.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
RFC5 Antibody (Center R185) (orb34152)
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