REV1L Antibody

• Catalog Number: orb1247029
• Category: Antibodies
• Description: REV1L Antibody
• Target: REV1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-VLQQTYGSTLKVT
• UniProt ID: Q9UBZ9
• Tested applications: ELISA, IHC
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: REV1, REV1L, REV1-like (yeast), REV1, REV1 protein
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_057400.1, NP_001032961.1

orb1247029 (4 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.