REV1 Antibody

SKU: orb1247029

Description

REV1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5µg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-VLQQTYGSTLKVT
• Target: REV1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

REV1, REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1 homolog (S. cerevisiae), FLJ21523, MGC163283, MGC26225, REV1- like

orb1247029 (4 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Quick Database Links

Gene Symbol

REV1

UniProt

Q9UBZ9

RefSeq (Protein):NP_001032961.1, NP_057400.1