RETN Antibody

• Catalog Number: orb1272664
• Category: Antibodies
• Description: RETN Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1.2_3D7-1D11
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Resistin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Resistin as the immunizing antigen.
• Concentration: batch dependent
• Dilution range: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μg/mL. Western Blot:To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: RETN
• UniProt ID: Q9HD89
• NCBI: Q9HD89
• Storage: Resistin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
• Alternative names: Adipocyte Secreted Factor, ADSF, FIZZ3, HXCP1, RST
• Note: For research use only
• Application notes: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 200 pg/mL of recombinant human Resistin when used with our biotinylated antigen affinity purified anti-human Resistin as the detection antibody at a concentration of at least 0.5-1.0 μg/mL. Western Blot:To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

To a sandwich ELISA (assuming 100 ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 200 pg/ml of recombinant human Resistin when used with biotinylated antigen affinity purified anti-human Resistin (orb1272327) as the detection antibody at a concentration of at least 0.5-1.0 ug/ml.

To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.

To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 0.20-0.40 ng/lane, under non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 ug/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.