RETN Antibody

• Catalog Number: orb1272328
• Category: Antibodies
• Description: RETN Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hResistin (human Resistin).
• Concentration: batch dependent
• Dilution range: ELISA:To detect hResistin by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hResistin. Sandwich:To detect hResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Resistinas a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.Western Blot:To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: RETN
• UniProt ID: Q9HD89
• NCBI: Q9HD89
• Storage: Resistin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: ADSF, RSTN, XCP1, FIZZ3, RETN1, HXCP1, UNQ407/PRO1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect hResistin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human Resistin (orb1272327) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hResistin.

To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 1.0 mg/ml with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.