RENT1 Antibody

• Catalog Number: orb1527585
• Category: Antibodies
• Description: RENT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1079 and 1129
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RENT1
• UniProt ID: Q92900
• NCBI: NP_002902.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: smg-2 homolog, nonsense mediated mRNA decay factor
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse RENT1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody (orb1527585) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 µg) from K-562, HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody (orb1527585) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody (orb1527585) used for IP at 4 µg per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (orb1527585) and a second antibody against a different epitope of RENT1.