You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb334574 |
---|---|
Category | Antibodies |
Description | RENT1/hUPF1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Predicted Reactivity | Gallus |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human RENT1/hUPF1 (578-614aa NMDSMPELQKLQQLKDETGELSSADEKRYRALKRT AE), identical to the related mouse and rat sequences. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 124345 MW |
UniProt ID | Q92900 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Regulator of nonsense transcripts 1;3.6.4.-;ATP-de Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of PC-3 cells using anti-RENT1/hUPF1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody.Lane 1:human HeLa cell; 2:human Raji cell; 3:human HepG2 cell;4:human SK-OV-3 cell;5:human PC-3 cell;6:human HEK293 cell;7:rat RH35 cell;8:mouse HEPA1-6 cell.
IF analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody.RENT1/hUPF1 was detected in immunocytochemical section of A431 cell.
IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in a paraffin-embedded section of mouse intestine tissue.
IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in a paraffin-embedded section of rat intestine tissue.
IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody. RENT1/hUPF1 was detected in a paraffin-embedded section of human intestinal cancer tissue.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
Filter by Rating