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Item 1 of 7
REG3G Antibody (Center)
Catalog Number: orb1930434
| Catalog Number | orb1930434 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB27078 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:100, WB: 1:1000, WB: 1:400, WB: 1:2000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 19330 Da |
| Target | This REG3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-116 amino acids of human REG3G. |
| UniProt ID | Q6UW15 |
| NCBI | NP_001256969.1, NP_001008388.1, NP_940850.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Regenerating islet-derived protein 3-gamma, REG-3- Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
REG3G Antibody (Center) western blot analysis in SH-SY5Y cell line lysates (35 ug/lane). This demonstrates the REG3G antibody detected the REG3G protein (arrow).
Western blot analysis of lysate from SH-SY5Y cell line, using REG3G Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.
Anti-REG3G Antibody (Center) at 1:400 dilution + human kidney lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-REG3G Antibody (Center) at 1:1000 dilution + recombinant protein lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-REG3G Antibody (Center) at 1:1000 dilution. Lane 1: mouse small intestine tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution. Observed band size: 20kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-REG3G Antibody (Center) at 1:2000 dilution. Lane 1: human kidney lysates. Lane 2: human lung lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-REG3G Antibody (Center) at 1:100 dilution. Lane 1: human kidney lysates. Lane 2: human pancreas lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 19 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
