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Catalog Number | orb668907 |
---|---|
Category | Proteins |
Description | Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting. |
Reactivity | Bacteria |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 13.8 kDa |
Target | Streptavidin |
Expression System | E. coli |
Biological Origin | Streptomyces avidinii |
Expression Region | Ala 37 - Ser 163 |
Endotoxins | 1.0 EU per μg |
Storage | -20°C to -70°C for 2 years in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Buffer/Preservatives | NaCl, pH 7.4 |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 13.8 kDa. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
18.5 kDa | |
Yeast |
Greater than 90% as determined by SDS-PAGE. | |
43.5 kDa | |
E.coli |
Greater than 98.0% as determined by SDS-PAGE and HPLC. | |
Escherichia Coli |