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Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine FDC-P1 is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x108 IU/mg. |
| Molecular Weight | Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| Protein Sequence | APTRSPNPVT RPWKHVDAIK EALSLLNDMR ALENEKNEDV DIISNEFSIQ RPTCVQTRLK LYKQGLRGNL TKLNGALTMI ASHYQTNCPP TPETDCEIEV TTFEDFIKNL KGFLFDIPFD CWKPVQK |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rRtGM-CSF as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Rat GM-CSF [orb3002300]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 15.6 KDa. Observed: 17-25 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRat CSF2 protein [orb707320]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
10 μg, 500 μg, 50 μgRecombinant Rat Granulocyte- Macrophage Colony Stimulating Factor (rrGM-CSF) [orb1494915]
>98% by SDS-PAGE and HPLC analyses.
Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.
Escherichia coli.
1 mg, 20 μg, 5 μgRecombinant Murine Granulocyte- Macrophage Colony Stimulating Factor (rMuGM-CSF) [orb1494926]
>95% by SDS-PAGE and HPLC analyses.
Recombinant murine GM-CSF is a 14.2 kDa globular protein consisting of 124 amino acids residues.
Escherichia coli.
1 mg, 100 μg, 5 μg

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Protocol Information
Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (orb1906122)
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