Recombinant Rat Granulocyte- Macrophage Colony Stimulating Factor (rrGM-CSF)

SKU: orb1494915

Description

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Images & Validation

• Application Notes:
  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

Key Properties

• Source: Escherichia coli.
• Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of Murine FDC-P1 cells is 1.0 x 108 units/mg.
• Molecular Weight: Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.
• Protein Sequence: MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK
• Purification: >98% by SDS-PAGE and HPLC analyses.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1EU/mg of rr GM-CSF as determined by LAL method.

Storage & Handling

• Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Form/Appearance: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
• Buffer/Preservatives: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
• Disclaimer: For research use only