Recombinant Protein G

• Catalog Number: orb257803
• Category: Proteins
• Description: Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.
• Reactivity: Bacteria
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 98%
• Conjugation: Unconjugated
• MW: 22.5 kDa
• Target: Protein G
• Source: Recombinant Protein G, His Tag (orb257803) is expressed from E. coli cells.
• Expression System: E. coli
• Endotoxins: 1.0 EU per μg
• Storage: -20°C
• Buffer/Preservatives: 100 mM NaH2PO4, pH8.5
• Alternative names: RPG,Protein G
• Note: For research use only
• Application notes: Recombinant Protein G, His Tag is fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. The reducing (R) protein migrates as a 29 kDa polypeptide. Protein G Protein can bind to human IgG1 Fc.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Protein G protein