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Catalog Number | orb257496 |
---|---|
Category | Proteins |
Description | Human G-CSF, premium grade is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Pro 204 (Accession # NP_757373.1). |
Target | G-CSF |
Conjugation | Unconjugated |
Tag | Native |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_757373.1 |
UniProt ID | P09919 |
MW | 18.7 kDa |
Application notes | This protein carries no "tag". The protein has a calculated MW of 18.7 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE). |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human G-CSF, premium grade (Cat. No. ) at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, ,His Tag (Cat. No. GCR-H82E4) with a linear range of 10-156 ng/mL (QC tested). |
Expression Region | Thr 31 - Pro 204 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_757373.1 |
SDS-PAGE analysis of Human G-CSF protein
Immobilized Human G-CSF, premium grade (Cat. No. orb257496) at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, Avitag, His Tag with a linear range of 10-156 ng/mL (QC tested).
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
> 98% as determined by SDS-PAGE and HPLC. | |
18.7 kDa | |
E.Coli |
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |