Human G-CSF Protein

• Catalog Number: orb257496
• Category: Proteins
• Description: Granulocyte colony-stimulating factor (G-CSF or GCSF) is also known as colony-stimulating factor 3, CSF3, C17orf33, CSF3OS, GCSF, MGC45931. It is a glycoprotein, growth factor and cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF regulates them using Janus kinase (JAK)/signal transducer and activator of transcription (STAT) and Ras /mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. G-CSF is produced by endothelium, macrophages, and a number of other immune cells. The natural human glycoprotein exists in two forms, a 174- and 180-amino-acid-long protein of molecular weight 19,600 grams per mole. G-CSF can effect on the hematopoietic system and neuronal cells as a neurotrophic factor. The action of G-CSF in the central nervous system is to induce neurogenesis, to increase the neuroplasticity and to counteract apoptosis. G-CSF stimulates the production of white blood cells (WBC). In oncology and hematology, a recombinant form of G-CSF is used with certain cancer patients to accelerate recovery from neutropenia after chemotherapy, allowing higher-intensity treatment regimens. Another form of recombinant human G-CSF called lenograstim is synthesised in Chinese Hamster Ovary cells (CHO cells). The recombinant human G-CSF synthesised in an E. coli expression system is called filgrastim.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 18.7 kDa
• Target: G-CSF
• Source: Human G-CSF, premium grade (orb257496) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Pro 204 (Accession # NP_757373.1).
• Expression System: HEK293
• Biological Activity: Immobilized Human G-CSF, premium grade at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, , His Tag with a linear range of 10-156 ng/mL (QC tested).
• Expression Region: Thr 31 - Pro 204
• Endotoxins: 0.1 EU per μg
• NCBI: NP_757373.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: G-CSF,CSF3,C17orf33,CSF3OS,MGC45931
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 18.7 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human G-CSF protein

Immobilized Human G-CSF, premium grade (Cat. No. orb257496) at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, Avitag, His Tag with a linear range of 10-156 ng/mL (QC tested).