Recombinant Murine Sonic Hedgehog

• Catalog Number: orb1905933
• Category: Proteins
• Description: Recombinant Murine Sonic Hedgehog
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• MW: Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
• UniProt ID: Q62226
• Protein Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 μg/ml.
• Endotoxins: Less than 1 EU/ugof rMuSHH as determined by LAL method.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Alternative names: SHH, HHG-1
• Note: For research use only
• Expiration Date: 6 months from date of receipt.