Recombinant IL-33, Human

• Catalog Number: orb1494819
• Category: Proteins
• Description: Interleukin-33 (IL-33) is a proinflammatory cytokine that belongs to the IL-1 family. IL-33 is expressed in a variety of cells, including epithelial and endothelial cells, smooth muscle cells, macrophages and fibroblasts. The primary receptors for IL-33 are ST2 and IL-1 receptor accessory protein (IL-1RAcP), both of which belong to the IL-1 receptor family. IL-33 is localized to the nucleus of resting cells where it binds to chromatin in the H2A-H2B histone complex as a transcriptional suppressor. IL-33 is secreted by cells during injury which induces a T-helper 2 type inflammatory response. Evidence suggests IL-33 plays a role in autoimmune disease. IL-33’s interaction with ST2 can drive allergic pathology and IL-33 has been reported to play a role in the development of rheumatoid arthritis and systemic lupus erythematosus.Recombinant humanInterleukin-33 (rhIL-33) produced in E.coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rhIL-33 has a molecular mass of 18.0 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• MW: 18.0 kDa, observed by reducing SDS-PAGE.
• Protein Sequence: SIT GISPITEYLA SLSTYNDQSI TFALEDESYE IYVEDLKKDE KKDKVLLSYY ESQHPSNESG DGVDGKMLMVTLSPTKDFWL HANNKEHSVE LHKCEKPLPD QAFFVLHNMH SNCVSFECKT DPGVFIGVKD NHLALIKVDS SENLCTENIL FKLSET
• Source: Escherichia coli.
• Biological Activity: ED50 2× 10ˆ6 units/mg.
• Endotoxins: < 0.2 EU/μg, determined by LAL method.
• Storage: Lyophilized recombinant human Interleukin-33 (rhIL-33) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-33 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
• Buffer/Preservatives: Lyophilized after extensive dialysis against PBS.
• Alternative names: Interleukin-33, IL33
• Note: For research use only
• Application notes: Reconstituted in ddH2O at 100 μg/mL.
• Expiration Date: 6 months from date of receipt.