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Catalog Number | orb391979 |
---|---|
Category | Proteins |
Description | Recombinant human IL33 protein is produced by our E.coli expression system and the target gene encoding Ser112-Thr270 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.25 |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL33 |
Entrez | 90865 |
UniProt ID | O95760 |
Protein Sequence | MGSSHHHHHH SSGLVPRGSH MSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.25 |
Alternative names | C9orf26; IL1F11; NFHEV; Interleukin-33; IL-33; Int Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
17.9 kDa | |
E.Coli |
Unconjugated | |
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. | |
20.2 kDa | |
Human IL-33, His Tag (orb612145) is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1). |
Unconjugated | |
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. | |
20.2 kDa | |
Cynomolgus IL-33, His Tag (orb668884) is expressed from human 293 cells (HEK293). It contains AA His 109 - Ile 270 (Accession # A0A2K5W3I1-1). |
Unconjugated | |
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. | |
19.5 kDa | |
Mouse IL-33, His Tag (orb750372) is expressed from human 293 cells (HEK293). It contains AA Ser 109 - Ile 266 (Accession # Q8BVZ5-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
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