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Catalog Number | orb1714858 |
---|---|
Category | Proteins |
Description | Recombinant Human TWEAK |
Tested applications | ELISA, WB |
Reactivity | Human |
Tag | N-terminal His Tag |
Purity | Greater than 90% by SDS-PAGE gel analyses |
MW | 19.2 kDa |
UniProt ID | O43508 |
Source | E.Coli |
Expression Region | 93-249 |
Storage | -20°C for 12 months as lyophilized; 2-8°C for 1 month under sterile conditions after reconstitution |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4 |
Alternative names | APO3 ligand, APO3L, DR3LG, TNFSF12, TWEAK Read more... |
Note | For research use only |
Application notes | Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 43.3 kDa after removal of the signal peptide. | |
Mammalian |
Greater than 90% as determined by SDS-PAGE. | |
38.9 kDa | |
E.coli |
98.00% | |
49.62 kDa (predicted). Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
Greater than 95% as determined by SDS-PAGE. | |
Escherichia coli |