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Catalog Number | orb1516370 |
---|---|
Category | Proteins |
Description | Recombinant human NKG2D protein, N-His-Avi (HEK293) |
Target | NKG2-D type II integral membrane protein |
Tag | N-His-Avi |
Form/Appearance | Lyophilized |
Concentration | >0.5 mg/ml |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Purity | > 95% as determined by Tris-Bis PAGE |
Protein Sequence | 78-216/216 |
MW | Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result. |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expression System | HEK293 cell |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Biological Activity | Not tested |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Alternative names | NKG2-D type II integral membrane protein; KLRK1; n Read more... |
Note | For research use only |
Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human NKG2D, His Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Anti-NKG2D Ab. with the EC50 of 5.3 ng/ml determined by ELISA.
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result. |
98.00% | |
19.0 kDa (predicted). Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result. |
98.00% | |
19.0 kDa (predicted). Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result. |