Recombinant Human Malic Enzyme 2, His

• Catalog Number: orb1905971
• Category: Proteins
• Description: Recombinant Human Malic Enzyme 2, His
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• MW: Approximately 64.4 kDa, a single non-glycosylated polypeptide chain containing 573 amino acids with 6 x His at C-terminus.
• UniProt ID: P23368
• Protein Sequence: MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RNTLIQFEDF GNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAAL GIANLIVMSM VENGLSEQEA QKKIWMFDKY GLLVKGRKAK IDSYQEPFTH SAPESIPDTF EDAVNILKPS TIIGVAGAGR LFTPDVIRAM ASINERPVIF ALSNPTAQAE CTAEEAYTLT EGRCLFASGS PFGPVKLTDG RVFTPGQGNN VYIFPGVALA VILCNTRHIS DSVFLEAAKA LTSQLTDEEL AQGRLYPPLA NIQEVSINIA IKVTEYLYAN KMAFRYPEPE DKAKYVKERT WRSEYDSLLP DVYEWPESAS SPPVITEHHH HHH
• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 �� 0.6 mM.
• Endotoxins: Less than 1 EU/ugof rHuME2, His as determined by LAL method.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Alternative names: NAD-dependent Malic Enzyme, Mitochondrial, NAD-ME
• Note: For research use only
• Expiration Date: 6 months from date of receipt.