Human GPC3

SKU: orb2995093

Description

Recombinant Human Glypican-3 is produced by our Mammalian expression system and the target gene encoding Gln25-His559 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human FGFb (146AA)(Cat. No.:orb3002475) at 2 μg/ml (100 μl/well) can bind Human GPC3 (C-6His)(Cat. No.:orb2995093). The EC50 of Human GPC3 (C-6His)(Cat. No.:orb2995093) is not higher than 50 ng/ml.
• Tag: C-6His
• Molecular Weight: Predicted: 61.6 KDa. Observed: 70-150&38 KDa, reducing conditions
• Expression Region: Gln25-His559
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS,4%Mannitol,pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glypican-3; GTR2-2; Intestinal protein OCI-5; MXR7; GPC3; OCI5

Quick Database Links

UniProt

P51654