Recombinant human Glypican 3 protein, C-His (HEK293)

• Catalog Number: orb1516441
• Category: Proteins
• Description: Recombinant human Glypican 3 protein, C-His (HEK293)
• Target: Glypican-3
• Tag: C-His
• Concentration: >0.5 mg/ml
• Purity: >95% as determined by Tris-Bis PAGE; >95% as determined by SEC-HPLC
• Purification: AC
• Protein Sequence: 25-559/580
• MW: Due to glycosylation, the protein migrates to 25, 42, 70-115 kDa based on Tris-Bis PAGE result.
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expression System: HEK293 cell
• Endotoxins: <1.0 EU/μg as determined by LAL
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Alternative names: GPC3; glypican3; glypican-3; Intestinal protein OC
• Research Area: Cancer Research, Cell Signaling, Developmental Bio
• Note: For research use only
• Expiration Date: 6 months from date of receipt.

Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Immobilized Human GPC3, His Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Anti-GPC3 Ab. with the EC50 of 11.7 ng/ml determined by ELISA.

Immobilized Human GPC3, His Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Anti-GPC3 Ab. with the EC50 of 11.7/11.3/14.3 ng/ml determined by ELISA.

The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.