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| Catalog Number | orb2992645 |
|---|---|
| Category | Proteins |
| Description | Recombinant Human Pituitary adenylate cyclase-activating polypeptide type I receptor is produced by our Mammalian expression system and the target gene encoding Met21-Ser416 is expressed with a 6His, Flag tag at C-terminus. VLPs are self-assembled from viral envelope/capsid protein and can display conformationally intact membrane protein in natural cell membranes. |
| Tag | C-6His-Flag |
| Form/Appearance | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4. |
| Purity | SEC-HPLC: Greater than 85% as determined by SEC-HPLC.(QC verified) |
| UniProt ID | P41586 |
| MW | Predicted: 48.2 KDa |
| Expression System | Human Cells |
| Endotoxins | < 1 EU/μg as determined by LAL test. |
| Biological Origin | Human |
| Biological Activity | ELISA: Immobilized Recombinant Human ADCYAP1R1 (VLP) (Cat#orb2992645) at 2 μg/ml (100 μl/well) can bind Anti-Human ADCYAP1R1 mab (Zelminemab). The ED50 of Anti-Human ADCYAP1R1 mab (Zelminemab) is 2.166 ng/ml. (Regularly tested). |
| Expression Region | Met21-Ser416 |
| Storage | Store at ≤ -70°C, stable for 6 months after receipt. Store at ≤ -70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Alternative names | Pituitary adenylate cyclase-activating polypeptide Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Human | |
0.16-10ng/mL | |
0.1 ng/mL |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 48.2 KDa. Observed: 55- 80 KDa, reducing conditions |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 16.7 KDa. Observed: 24- 40 KDa, reducing conditions |
SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) | |
Predicted: 48.2 KDa |
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE. (QC verified) | |
Predicted: 42.6 KDa. Observed: 50-70 KDa, reducing conditions |
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