Recombinant Granzyme B Antibody

• Catalog Number: orb2635472
• Category: Antibodies
• Description: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: GZMB/4539R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 73-187 was used as the immunogen for the recombinant Granzyme B antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Granzyme B antibody is available for research use only.
• UniProt ID: P10144
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Granzyme B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human spleen tissue lysate using recombinant Granzyme B antibody (clone GZMB/4539R). Predicted molecular weight: 29-37 kDa.

IHC staining of FFPE human spleen tissue with recombinant Granzyme B antibody (clone GZMB/4539R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant Granzyme B antibody (clone GZMB/4539R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Granzyme B antibody (clone GZMB/4539R) as confirmation of integrity and purity.