Recombinant Cyn. monkey CD3E & CD3G protein, C-hFc (HEK293)

• Catalog Number: orb1516501
• Category: Proteins
• Description: Recombinant Cyn. monkey CD3E & CD3G protein, C-hFc (HEK293)
• Target: T-cell surface glycoprotein CD3 epsilon chain
• Tag: C-hFc
• Form/Appearance: Lyophilized
• Concentration: ＞0.5 mg/ml
• Buffer/Preservatives: Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
• Protein Sequence: 20-198/198
• MW: Due to glycosylation,the protein migrates to 44-46 & 46-48 kDa based on Tris-Bis PAGE result.
• Application notes: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Expression System: HEK293 cell
• Endotoxins: < 1.0 EU/μg as determined by LAL
• Biological Activity: Not tested
• Storage: Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
• Alternative names: CD3; CD3D; CD3-DELTA; T3D; CD3G;Recombinant Cyn. m
• Note: For research use only

Immobilized Cyno CD3 Epsilon & CD3 Gamma, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Biotinylated SP34, hFc Tag with EC50 of 14.0 ng/ml /14.6 ng/ml /13.6 ng/ml determined by ELISA.

Immobilized Cyno CD3 Epsilon & CD3 Gamma, hFc Tag at 0.5 µg/ml (100 µl/Well). Dose response curve for Biotinylated SP34, hFc Tag with EC50 of 14.0 ng/ml determined by ELISA.

The purity of Cynomolgus CD3E&CD3G / CD3 Epsilon&CD3 Gamma was greater than 95% as determined by SEC-HPLC.

ynomolgus CD3E&CD3G / CD3 Epsilon&CD3 Gamma on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.