Recombinant Canine Tumor Necrosis Factor-alpha/TNFSF2

SKU: orb1906144

Description

Recombinant Canine Tumor Necrosis Factor-alpha/TNFSF2

Research Area

Cancer Biology; Immunology & Inflammation

Key Properties

• Source: Escherichia coli
• Expression System: Escherichia coli
• Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x106 IU/mg in the presence of actinomycin D.
• Molecular Weight: Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
• Protein Sequence: VKSSSRTPSD KPVAHVVANP EAEGQLQWLS RRANALLANG VELTDNQLIV PSDGLYLIYS QVLFKGQGCP STHVLLTHTI SRFAVSYQTK VNLLSAIKSP CQRETPEGTE AKPWYEPIYL GGVFQLEKGD RLSAEINLPN YLDFAESGQV YFGIIAL
• Purity: > 95 % by SDS-PAGE and HPLC analyses.
• Endotoxins: Less than 1 EU/ug of rCaTNF-α as determined by LAL method.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Form/Appearance: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P51742