Recombinant Calnexin (Endoplasmic Reticulum Marker) Antibody

• Catalog Number: orb2317141
• Category: Antibodies
• Description: Recombinant Calnexin (Endoplasmic Reticulum Marker) Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: CANX/9147R
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Dilution range: ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 90kDa
• Target: CANX
• Entrez: 821
• UniProt ID: P27824
• Expression System: CHO cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Calnexin; CANX; CNX; IP90; Major histocompatibilit
• Note: For research use only
• Application notes: Positive Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
• Expiration Date: 12 months from date of receipt.