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Catalog Number | orb1494905 |
---|---|
Category | Proteins |
Description | Betacellulin (BTC) is a member of the EGF family of growth factors that also includes EGF, TGF-α, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin, Heregulin and Neuregulins. Mature human BTC protein exhibits 80% amino acidsimilarity with mouse BTC protein. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. It is synthesized primarily as a transmembrane precursor, which is then processed to a mature molecule by proteolytic events. BTC signals through the EGF receptor.Recombinant Human Betacellulin (BTC) produced in E.coli is a single non-glycosylated polypeptide chain containing 81 amino acids. A fully biologically active molecule, rhBTC has a molecular mass of 15 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
MW | 15 kDa, observed by reducing SDS-PAGE. |
Protein Sequence | MDGNSTRSP ETNGLLCGDP EENCAATTTQ SKRKGHFSRC PKQYKHYCIK GRCRFVVAEQ TPSCVCDEGY IGARCERVDL FY |
Source | Escherichia coli. |
Biological Activity | The ED50 was determined by the dose-dependent stimulation of the proliferation of murine Balb/3T3 cells is 1 x 10ˆ8 units/mg. |
Endotoxins | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Betacellulin (BTC) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human Betacellulin (BTC) should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Buffer/Preservatives | Lyophilized after extensive dialysis against PBS. |
Alternative names | BTC Read more... |
Note | For research use only |
Application notes | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Expiration Date | 6 months from date of receipt. |
> 98 % by SDS-PAGE and HPLC analyses. | |
Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. | |
Escherichia coli |
Greater than 90% as determined by SDS-PAGE. | |
43.6 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
18.6 kDa | |
Yeast |
SDS-PAGE | |
Unconjugated | |
> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
HPLC, SDS-PAGE | |
Unconjugated | |
> 96% by SDS-PAGE and HPLC analyses | |
9.0 kDa |
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