RECK Rabbit Polyclonal Antibody

• Catalog Number: orb6867
• Category: Antibodies
• Description: RECK Rabbit Polyclonal Antibody
• Target: RECK
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human RECK (801-900/971aa)
• UniProt ID: O95980
• RRID: AB_10939539
• MW: 107 kDa
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: hRECK; Membrane anchored glycoprotein(metastasis a
• Research Area: Cancer Research, Tumor Suppressors
• Note: For research use only

Blank control: U937. Primary Antibody (green line): Rabbit Anti-RECK antibody (orb6867), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells wereincubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Mouse NIH/3T3 cell lysates, Lane 3: Human Huvec cell lysates, Lane 4: Human U87MG cell lysates, Primary: Anti-RECK (orb6867) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 107 kDa, Observed band size: 120 kDa.