RBPJK Rabbit Polyclonal Antibody

SKU: orb101350

Description

RBPJK Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RBPJK (101-200/500aa)
• Target: RBPJ
• Molecular Weight: 56 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AI843960; CBF 1; CBF-1; CBF1; IGKJRB1; J kappa recombination signal binding protein; J kappa-recombination signal binding protein; J kappa-recombination signal-binding protein; KBF2; NY REN 30 antigen; RBP J kappa; RBP-J; RBP-J kappa; RBP-JK; Rbpj; RBPJK; RBPSUH; Recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RBPJK) Polyclonal Antibody, Unconjugated (orb101350) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RBPJK) Polyclonal Antibody, Unconjugated (orb101350) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Embryo (Mouse) Lysate at 40 ug, Colon carcinoma (Human) Lysate at 40 ug, Primary: Anti-RBPJK (orb101350) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution.

Sample: Embryo (Mouse) Lysate at 40 ug, Primary: Anti-RBPJK (orb101350) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Quick Database Links

Gene Symbol

RBPJ

UniProt

Q06330

References

Qiang Du 1, Chun Zhang 1, Tianyu Qu 1, Xiao Zhou 1, Yingying Liu 1, Zhixuan Chen 1, Zilin Shen 1, Pingsheng Chen 2, Ruifeng Zhang METTL3-Driven m6A Modification of RBPJ Promotes Vascular Remodeling in Pulmonary Hypertension Am J Pathol, (2024)

PubMed: 39222906

Applications

IF

Reactivity

Mouse