RBPJK Rabbit Polyclonal Antibody

• Catalog Number: orb101350
• Category: Antibodies
• Description: RBPJK Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RBPJK (101-200/500aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: RBPJ
• UniProt ID: Q06330
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AI843960; CBF 1; CBF-1; CBF1; IGKJRB1; J kappa rec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Qiang Du 1, Chun Zhang 1, Tianyu Qu 1, Xiao Zhou 1, Yingying Liu 1, Zhixuan Chen 1, Zilin Shen 1, Pingsheng Chen 2, Ruifeng Zhang METTL3-Driven m6A Modification of RBPJ Promotes Vascular Remodeling in Pulmonary Hypertension Am J Pathol, (2024)

PubMed: 39222906

Applications

IF

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RBPJK) Polyclonal Antibody, Unconjugated (orb101350) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RBPJK) Polyclonal Antibody, Unconjugated (orb101350) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Embryo (Mouse) Lysate at 40 ug, Colon carcinoma (Human) Lysate at 40 ug, Primary: Anti-RBPJK (orb101350) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution.

Sample: Embryo (Mouse) Lysate at 40 ug, Primary: Anti-RBPJK (orb101350) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.