RBP1 Antibody

• Catalog Number: orb1252707
• Category: Antibodies
• Description: RBP1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: G4E4
• Tested applications: IF, IHC, WB
• Reactivity: Goat, Human, Monkey, Mouse, Rabbit, Rat
• Isotype: IgG1, kappa
• Immunogen: Retinol binding protein purified from human plasma was used as the immunogen for this RBP antibody. Its epitope localizes between amino acids 74-182.
• Concentration: 0.2 mg/mL
• Dilution range: IHC (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)IF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RBP1
• UniProt ID: P09455
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: RBP1, CRABP-I, CRBP, CRBP1, RBPC, CRBPI
• Note: For research use only
• Application notes: IHC (FFPE): 1-2 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)IF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human hepatocellular carcinoma and RBP antibody (clone G4E4).

SDS-PAGE Analysis of Purified, BSA-Free RBP Antibody (clone G4E4). Confirmation of Integrity and Purity of the Antibody.