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Catalog Number | orb392698 |
---|---|
Category | Proteins |
Description | Recombinant rat VEGFA protein is produced by our yeast expression system and the target gene encoding Ala27-Arg190 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Rat |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | VEGFA |
Entrez | 83785 |
UniProt ID | P16612 |
Protein Sequence | APTTEGEQKT HEVVKFMDVY QRSYCRPIET LVDIFQEYPD EIEYIFKPSC VPLMRCAGCC NDEALECVPT SESNVTMQIM RIKPHQSQHI GEMSFLQHSR CECRPKKDRT KPENHCEPCS ERRKHLFVQD PQTCKCSCKN TDSRCKARQL ELNERTCRCD KPRR |
Source | P. pastoris |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | VEGF; Vascular endothelial growth factor A; VEGF-A Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
26.1 kDa | |
E.coli |
> 95% by SDS-PAGE. | |
Recombinant Rat VEGFA/VEGF164 Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Ala27-Arg190) of Rat VEGFA (Accession #NP_001274037.1) fused with an 6��His Tag at the C-terminus. |
> 86%, determined by SDS-PAGE | |
This protein contains the rat VEGF 164 isoform (AAA41211.1) (Met 1-Arg 190) was expressed. |