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Catalog Number | orb420188 |
---|---|
Category | Proteins |
Description | Rat Transferrin protein |
Tested applications | ELISA, IHC, WB |
Isotype | Transferrin |
Concentration | 5.5 mg/ml |
Dilution range | ELISA: User Optimized, IHC: User Optimized, WB: User Optimized |
Form/Appearance | Liquid (sterile filtered) |
Purity | Rat Transferrin was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Rat Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Transferrin and anti-Rat Serum. |
Conjugation | Unconjugated |
Target | Tf |
UniProt ID | P12346 |
Source | Rat |
NCBI | AAH87021.1 |
Storage | Store vial at 4° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. RAT TRANSFERRIN DOES NOT CONTAIN PRESERVATIVE. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 |
Alternative names | Tfn, Trf, MGC93500, Serotransferrin Read more... |
Note | For research use only |
Application notes | Rat Transferrin is a suitable protein for use as a control reagent in both Western Blotting and ELISA experiments. For blotting, the expected molecular weight of Rat Transferrin is approximately 80kDa. |
Expiration Date | 6 months from date of receipt. |
ELISA, SDS-PAGE, WB | |
> 90%(SDS-PAGE) | |
Purified frome rat serum. |
Greater than 85% as determined by SDS-PAGE. | |
76.0 kDa | |
Yeast |
Greater than 85% as determined by SDS-PAGE. | |
89.9 kDa | |
E.coli |
Greater than 85% as determined by SDS-PAGE. | |
83.8 kDa | |
E.coli |
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