Rat IL6 protein

• Catalog Number: orb80065
• Category: Proteins
• Description: Rat IL6 protein
• Target: Rat IL6
• Conjugation: Unconjugated
• Buffer/Preservatives: The protein was lyophilized without any additives.
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
• Tested applications: FA, HPLC, SDS-PAGE
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-6 as Reference Standard.
• Biological Origin: E.coli
• Biological Activity: The ED50= 0.03-0.1 ng/mL. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications.
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Interleukin BSF 2 protein, B cell differentiation
• Note: For research use only