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Rat HER2 protein

SKU: orb391677

Description

Recombinant rat HER2 protein is produced by our E. coli expression system and the target gene encoding Ala67-Val323 is expressed with a 6His tag at the C-terminus.

Research Area

Cancer, Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityRat
Protein SequenceMANASLSFLQ DIQEVQGYML IAHNQVKRVP LQRLRIVRGT QLFEDKYALA VLDNRDPQDN VAASTPGRTP EGLRELQLRS LTEILKGGVL IRGNPQLCYQ DMVLWKDVFR KNNQLAPVDI DTNRSRACPP CAPACKDNHC WGESPEDCQI LTGTICTSGC ARCKGRLPTD CCHEQCAAGC TGPKHSDCLA CLHFNHSGIC ELHCPALVTY NTDTFESMHN PEGRYTFGAS CVTTCPYNYL STEVGSCTLV CPPNNQEVHH HHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 4M Urea, pH 8.0.
DisclaimerFor research use only

Alternative Names

HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Rat HER2 protein (orb391677)

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Available Sizes

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10 μg
$ 260.00
50 μg
$ 600.00
500 μg
$ 4,020.00
DispatchUsually dispatched within 5-10 working days
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