Rat HER2 protein

SKU: orb391677

Description

Recombinant rat HER2 protein is produced by our E. coli expression system and the target gene encoding Ala67-Val323 is expressed with a 6His tag at the C-terminus.

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: ERBB2
• Reactivity: Rat
• Protein Sequence: MANASLSFLQ DIQEVQGYML IAHNQVKRVP LQRLRIVRGT QLFEDKYALA VLDNRDPQDN VAASTPGRTP EGLRELQLRS LTEILKGGVL IRGNPQLCYQ DMVLWKDVFR KNNQLAPVDI DTNRSRACPP CAPACKDNHC WGESPEDCQI LTGTICTSGC ARCKGRLPTD CCHEQCAAGC TGPKHSDCLA CLHFNHSGIC ELHCPALVTY NTDTFESMHN PEGRYTFGAS CVTTCPYNYL STEVGSCTLV CPPNNQEVHH HHHH
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 4M Urea, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340

Quick Database Links

Gene Symbol

ERBB2

UniProt

P06494

NCBI Gene ID

24337