Rat GCSF protein

• Catalog Number: orb80033
• Category: Proteins
• Description: Granulocyte MCSF Rat Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. GMCSF Rat Recombinant is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 95.0% as determined by(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE.
• Conjugation: Unconjugated
• Target: rGM CSF
• Solubility (25°C): It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.
• Biological Origin: E.coli
• Biological Activity: The ED50 range 0.001-0.01 ng/ml, determined by the dose dependent proliferation of murine MC/9 cells.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: GM-CSF Rat was lyophilized with no additives.
• Alternative names: CSF-2 protein, MGI-1GM protein, GM-CSF protein, Pl
• Note: For research use only
• Application notes: < strong>Experiment Notes: GMCSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.966 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated the PC GENE computer analysis program of protein sequences (telliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of GM-CSF as Reference Standard.
• Expiration Date: 6 months from date of receipt.