RASSF1A Rabbit Polyclonal Antibody

• Catalog Number: orb500734
• Category: Antibodies
• Description: RASSF1A Rabbit Polyclonal Antibody
• Target: RASSF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human RASSF1A (251-344/344aa)
• UniProt ID: Q9NS23
• MW: 39 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 123F2; AA536941; AU044980; D4Mgi37; MGC94319; NORE
• Research Area: Apoptosis, Cancer Research, Cell Biology, Cell Cyc
• Note: For research use only

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (RASSF1A) polyclonal Antibody, Unconjugated (orb500734) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (RASSF1A) Polyclonal Antibody, Unconjugated (orb500734) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Positive control: A549 cells (fixed with 70% ice-cold methanol overnight at 4°C, permeabilized with 0.1% PBS-Tween for 20 min at room temperature), Primary Antibody: Rabbit Anti-RASSF1A antibody (orb500734) green line, Concentration: 1 ug/Test, Isotype Control Antibody: Rabbit IgG, Secondary Antibody: F (ab')2 fragment goat anti-rabbit IgG-FITC.

Sample: Lane 1: Thyroid gland (Mouse) Lysate at 40 ug, Lane 2: Kidney (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-RASSF1A (orb500734) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 40 kD, Observed band size: 42 kD.