RASIP1 Antibody

• Catalog Number: orb1263611
• Category: Antibodies
• Description: RASIP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This RASIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-678 amino acids from the Central region of human RASIP1.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 103 kDa
• Target: RASIP1
• UniProt ID: Q5U651
• NCBI: Q5U651
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ras-interacting protein 1, Rain, RASIP1
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing K562 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of RASIP1 Antibody in K562 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.